|
KMID : 1036720180510060507
|
|
Journal of Nutrition and Health
2018 Volume.51 No. 6 p.507 ~ p.514
|
|
|
Immunomodulating activity of Sargassum horneri extracts in RAW264.7 macrophages
|
|
Kim Dong-Sub
Sung Nak-Yun
Park Sang-Yun
Kim Geon
Eom Ji
Yoo Jin-Gon
Seo In-Ra
Han In-Jun
Cho Young-Baik
Kim Kyung-Ah
|
|
|
Abstract
|
|
|
Purpose: Sargassum horneri (S. horneri) is a species of brown macroalgae that is common along the coast of Japan and Korea. The present study investigated the immuno-modulatory effects of different types of S. horneri extracts in RAW264.7 macrophages.
Methods: S. horneri was extracted by three different methods, hot water extraction, 50% ethanol extraction, and supercritical fluid extraction. Cell viability was then measured by MTT assay, while the production levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and nitric oxide (NO) were measured by enzyme-linked immunosorbent assay and Griess assay, respectively. The expression and activation levels of inducible NO synthase (iNOS), mitogen-activated protein kinase (MAPK) and nuclear factor κB (NF-κB) were examined by western blot analysis.
Results: The three different S. horneri extracts were nontoxic against RAW 264.7 cells up to 50 μg/mL, among which treatment with hot water extract (HWE) of S. horneri significantly enhanced the production of TNF-α, IL-6, and NO in a dose-dependent manner. Hot water extract of S. horneri also increased the expression level of iNOS, suggesting that up-regulation of iNOS expression by HWE of S. horneri was responsible for the induction of NO production. In addition, treatment of RAW 264.7 macrophages with HWE of S. horneri increased the phosphorylation levels of ERK, p38 and JNK. Furthermore, the activation and subsequent nuclear translocation of NF-κB was enhanced upon treatment with HWE of S. horneri, indicating that HWE of S. horneri activates macrophages to secrete TNF-α, IL-6 and NO and induces iNOS expression via activation of the NF-κB and MAPKs signaling pathways.
Conclusion: Taken together, these findings suggest that HWE of S. horneri possesses potential as a functional food with immunomodulatory activity.
|
|
|
KEYWORD
|
|
|
Sargassum horneri, macrophages, nitric oxide, cytokines, MAPKs
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|